Plasmid_Backbone

Part:BBa_J428383:Design

Designed by: Vinoo Selvarajah   Group: 2021 Engineering Committee   (2022-06-15)


Loop pOdd-3 vector based on pJUMP26. Origin p15A (medium copy number)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 2292
    Illegal suffix found at 12
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2292
    Illegal SpeI site found at 13
    Illegal PstI site found at 27
    Illegal NotI site found at 20
    Illegal NotI site found at 2298
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2292
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2292
    Illegal suffix found at 13
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2292
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2307
    Illegal SpeI site found at 13
    Illegal PstI site found at 27
    Illegal NgoMIV site found at 1386
    Illegal AgeI site found at 1824
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 2314
    Illegal SapI.rc site found at 5


Design Notes

Marcos Valenzuela-Ortega, Christopher French, Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology, Synthetic Biology, Volume 6, Issue 1, 2021, ysab003, https://doi.org/10.1093/synbio/ysab003


Source

Marcos Valenzuela-Ortega, Christopher French, Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology, Synthetic Biology, Volume 6, Issue 1, 2021, ysab003, https://doi.org/10.1093/synbio/ysab003

References